Plasmid

Part:BBa_K4730002

Designed by: Sanvi Garg   Group: iGEM23_WVHS-SanDiego   (2023-10-04)


Level 1 PETase plasmid

This is a level one plasmid with the PETase gene sequence. It has a T7 promoter and the EF-rrnB terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1175
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 992
    Illegal SapI.rc site found at 1291


[edit]
Categories
Parameters
None